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Hack my GI Effects Test if you like

Discussion in 'Optimal Labs' started by vkiernan, Aug 6, 2012.

  1. vkiernan

    vkiernan Silver

    Had to do it as txt cuz the pdf was too big. Thanks much for any input.





    Age: 49 Sex: Female

    7/13/12

    Report Date:

    Date Received: 7/17/12





    2100 Gastrointestinal Function Profile

    Methodology: DNA Analysis, GC/MS, Microscopic,

    Colorimetric, Automated Chemistry, ELISA

    20% 40% 60% 80%

    Results 1st 2nd 3rd 4th 5th

    CFU/gram

    95%

    Reference

    Range

    Percentile Ranking by Quintile Consistency = Formed/Normal

    Predominant Bacteria (E+007) E+007

    Units and Reference Ranges

    Organisms are detected by DNA analysis.

    One colony forming unit (CFU) is equivalent to

    one bacterium. Each genome detected

    represents one cell, or one CFU. Results are

    expressed in scientific notation, so an organism

    reported as 2.5 E7 CFU/gram is read as 25

    million colony forming units per gram of feces.

    The cutoff for significance of Opportunistic

    Bacteria has been set at 1.0E+ 005 (100,000).

    These are levels above which clinically

    significant growth may be present. Rather than

    reporting semi-quantitative +1 to +4 levels, the

    new methodology provides full quantitative

    analysis.

    Predominant Bacteria play major roles in

    health. They provide colonization resistance

    against potentially pathogenic organisms, aid in

    digestion and absorption, produce vitamins and

    SCFA's, and stimulate the GI immune system.

    DNA probes allow detection of multiple species

    (sp.) within a genus, so the genera that are

    reported cover many species.



    Obligate anaerobes

    2.3 >= 1.3 1.6 6.7 Bacteroides sp. ®

    4.1 >= 1.0 1.5 6.2 Clostridia sp. ®

    2.2 >= 1.1 1.6 6.2 Prevotella sp. ®

    2.7 >= 1.1 1.6 7.4 Fusobacteria sp. ®

    2.1 >= 1.0 1.6 5.8 Streptomyces sp. ®

    5.7 >= 1.2 1.7 6.2 Mycoplasma sp. ®



    Facultative anaerobes

    3.1 >= 1.2 1.8 7.8 Lactobacillus sp. ®

    4.1 >= 1.8 2.3 7.6 Bifidobacter sp. ®



    Obligate aerobes

    5.3 >= 1.1 1.7 7.7 Escherichia coli (E. coli) ®



    Opportunistic Bacteria

    No clinically significant amounts.



    Opportunistic Bacteria may cause

    symptoms and be associated with disease.

    They can affect digestion and absorption,

    nutrient production, pH and immune state.

    Antibiotic sensitivity tests will be performed on

    all opportunistic bacteria found, although

    clinical history is usually considered to

    determine treatment since the organisms are

    not generally considered to be pathogens.

    Page 1





    Pathogenic Bacteria

    95% Reference

    Range

    Helicobacter pylori to even low rates of

    yeast growth. Potential symptoms include

    diarrhea, headache, bloating, atopic dermatitis

    and fatigue. Positives are reported as +1, +2,

    +3 or +4 indicating >100, >1000, >10000 or

    >100000 pg DNA/g.



    Parasites Expected Value

    Parasite present; taxonomy unavailable. Positive Neg

    A taxonomy unavailable finding likely indicates an ingested protozoan and not a human parasite. It

    does not indicate treatment unless patient symptoms and other inflammatory markers are consistent

    with parasite infection.

    Parasites

    Parasite infections are a major cause of

    non-viral diarrhea. Symptoms may include

    constipation, gas, bloating, increased allergy

    response, colitis, nausea and distention.



    Adiposity Index

    Firmicutes 74 ® = 20

    The Adiposity Index is derived by using

    DNA probes that detect multiple genera of the

    phyla Firmicutes and Bacteroidetes.

    Abnormalities of these phyla may be associated

    with increased caloric extraction from food.



    Drug Resistance Genes

    aacA, aphD Neg gyrB, ParE Neg

    mecA Neg PBP1a, 2B Neg

    vanA, B, and C Neg

    Drug Resistance Genes

    aacA, aphD - Gentamycin, Kanamycin, and

    Tobramycin

    mecA - Methicillin

    VanA, vanB, vanC - Vancomycin and Teicoplanin

    GyrB, ParE - Ciprofoxacin and later quinolones

    PBP1a, PBP2B - Penicillin

    Page 2



    2100 Gastrointestinal Function Profile

    Methodology: DNA Analysis, GC/MS, Microscopic,

    Colorimetric, Automated Chemistry, ELISA

    Percentile Ranking by Quintile

    1st 2nd 3rd 4th 5th

    20% 40% 60% 80%

    Results 95%

    Reference Range

    Beneficial SCFA

    Beneficial SCFA

    Short chain fatty acids (SCFA) are

    produced by bacterial fermentation of dietary

    polysaccharides and fiber. The product,

    N-butyrate, is taken up and used to sustain the

    normal activity of colonic epithielial cells.

    Butyrate has been shown to lower the risk of

    colitis and colorectal cancer. A healthy balance

    of GI microbes depends on production of SCFA

    by one specie to allow the normal growth of

    another one in a complex cross-feeding

    network.

    Total SCFA 34 L >= 35 53® mM/g

    n-Butyrate 5.3 >= 3.9 5.2® mM/g

    Acetate % 65 47 - 77 71® % 52

    Butyrate % 16 7 - 30 25® % 10

    Propionate % 18 10 - 29 25® % 13

    Valerate % 1.3 0.4 - 4.6 3.6 ® % 1.0



    Inflammation

    Inflammation

    Lactoferrin, an iron-binding glycoprotein,

    is released in IBD but not in non-inflammatory

    IBS. High levels are found in Crohn's, UC or

    infection. WBC's are elevated in general

    inflammation/infection. Mucus is often

    visualized in acute GI inflammation.

    Lactoferrin 2.3 ® or other microbes. Low sIgA

    can result from stress or malnutrition.

    Anti-gliadin sIgA is a screening marker for

    gluten sensitivity.

    Fecal sIgA 25 5 - 161 144 ® mg/dl 20

    Anti-gliadin sIgA 7.1 H 100 ug/g 200

    Triglycerides 100 ®
     
  2. vkiernan

    vkiernan Silver

    The txt file is almost impossible to read. I will have to clean it up somehow first.
     
  3. vkiernan

    vkiernan Silver

    I attemped to clean it up. Starts with my results then the range and then the max/min range.



    Anyone know how to post the actual pdf file?
     
  4. JanSz

    JanSz Gold

    vkiernan;43623 wrote: Had to do it as txt cuz the pdf was too big. Thanks much for any input.





    Age: 49 Sex: Female

    7/13/12

    Report Date:

    Date Received: 7/17/12





    2100 Gastrointestinal Function Profile

    Methodology: DNA Analysis, GC/MS, Microscopic,

    Colorimetric, Automated Chemistry, ELISA

    20% 40% 60% 80%

    Results 1st 2nd 3rd 4th 5th

    CFU/gram

    95%

    Reference

    Range

    Percentile Ranking by Quintile Consistency = Formed/Normal

    Predominant Bacteria (E+007) E+007

    Units and Reference Ranges

    Organisms are detected by DNA analysis.

    One colony forming unit (CFU) is equivalent to

    one bacterium. Each genome detected

    represents one cell, or one CFU. Results are

    expressed in scientific notation, so an organism

    reported as 2.5 E7 CFU/gram is read as 25

    million colony forming units per gram of feces.

    The cutoff for significance of Opportunistic

    Bacteria has been set at 1.0E+ 005 (100,000).

    These are levels above which clinically

    significant growth may be present. Rather than

    reporting semi-quantitative +1 to +4 levels, the

    new methodology provides full quantitative

    analysis.

    Predominant Bacteria play major roles in

    health. They provide colonization resistance

    against potentially pathogenic organisms, aid in

    digestion and absorption, produce vitamins and

    SCFA's, and stimulate the GI immune system.

    DNA probes allow detection of multiple species

    (sp.) within a genus, so the genera that are

    reported cover many species.



    Obligate anaerobes

    2.3 >= 1.3 1.6 6.7 Bacteroides sp. ®

    4.1 >= 1.0 1.5 6.2 Clostridia sp. ®

    2.2 >= 1.1 1.6 6.2 Prevotella sp. ®

    2.7 >= 1.1 1.6 7.4 Fusobacteria sp. ®

    2.1 >= 1.0 1.6 5.8 Streptomyces sp. ®

    5.7 >= 1.2 1.7 6.2 Mycoplasma sp. ®



    Facultative anaerobes

    3.1 >= 1.2 1.8 7.8 Lactobacillus sp. ®

    4.1 >= 1.8 2.3 7.6 Bifidobacter sp. ®



    Obligate aerobes

    5.3 >= 1.1 1.7 7.7 Escherichia coli (E. coli) ®



    Opportunistic Bacteria

    No clinically significant amounts.



    Opportunistic Bacteria may cause

    symptoms and be associated with disease.

    They can affect digestion and absorption,

    nutrient production, pH and immune state.

    Antibiotic sensitivity tests will be performed on

    all opportunistic bacteria found, although

    clinical history is usually considered to

    determine treatment since the organisms are

    not generally considered to be pathogens.

    Page 1





    Pathogenic Bacteria

    95% Reference

    Range

    Helicobacter pylori tigenic

    responses in some patients to even low rates of

    yeast growth. Potential symptoms include

    diarrhea, headache, bloating, atopic dermatitis

    and fatigue. Positives are reported as +1, +2,

    +3 or +4 indicating >100, >1000, >10000 or

    >100000 pg DNA/g.



    Parasites Expected Value

    Parasite present; taxonomy unavailable. Positive Neg

    A taxonomy unavailable finding likely indicates an ingested protozoan and not a human parasite. It

    does not indicate treatment unless patient symptoms and other inflammatory markers are consistent

    with parasite infection.

    Parasites

    Parasite infections are a major cause of

    non-viral diarrhea. Symptoms may include

    constipation, gas, bloating, increased allergy

    response, colitis, nausea and distention.



    Adiposity Index

    Firmicutes 74 ® = 20

    The Adiposity Index is derived by using

    DNA probes that detect multiple genera of the

    phyla Firmicutes and Bacteroidetes.

    Abnormalities of these phyla may be associated

    with increased caloric extraction from food.



    Drug Resistance Genes

    aacA, aphD Neg gyrB, ParE Neg

    mecA Neg PBP1a, 2B Neg

    vanA, B, and C Neg

    Drug Resistance Genes

    aacA, aphD - Gentamycin, Kanamycin, and

    Tobramycin

    mecA - Methicillin

    VanA, vanB, vanC - Vancomycin and Teicoplanin

    GyrB, ParE - Ciprofoxacin and later quinolones

    PBP1a, PBP2B - Penicillin

    Page 2



    2100 Gastrointestinal Function Profile

    Methodology: DNA Analysis, GC/MS, Microscopic,

    Colorimetric, Automated Chemistry, ELISA

    Percentile Ranking by Quintile

    1st 2nd 3rd 4th 5th

    20% 40% 60% 80%

    Results 95%

    Reference Range

    Beneficial SCFA

    Beneficial SCFA

    Short chain fatty acids (SCFA) are

    produced by bacterial fermentation of dietary

    polysaccharides and fiber. The product,

    N-butyrate, is taken up and used to sustain the

    normal activity of colonic epithielial cells.

    Butyrate has been shown to lower the risk of

    colitis and colorectal cancer. A healthy balance

    of GI microbes depends on production of SCFA

    by one specie to allow the normal growth of

    another one in a complex cross-feeding

    network.

    Total SCFA 34 L >= 35 53® mM/g

    n-Butyrate 5.3 >= 3.9 5.2® mM/g

    Acetate % 65 47 - 77 71® % 52

    Butyrate % 16 7 - 30 25® % 10

    Propionate % 18 10 - 29 25® % 13

    Valerate % 1.3 0.4 - 4.6 3.6 ® % 1.0



    Inflammation

    Inflammation

    Lactoferrin, an iron-binding glycoprotein,

    is released in IBD but not in non-inflammatory

    IBS. High levels are found in Crohn's, UC or

    infection. WBC's are elevated in general

    inflammation/infection. Mucus is often

    visualized in acute GI inflammation.

    Lactoferrin 2.3 ® style="color: #FF0000;">High fecal sIgA indicates immune system

    reactions to the presence of antigens from

    bacteria, yeast or other microbes. Low sIgA

    can result from stress or malnutrition.

    Anti-gliadin sIgA is a screening marker for

    gluten sensitivity.

    Fecal sIgA 25 5 - 161 144 ® mg/dl 20

    Anti-gliadin sIgA 7.1 H 100 ug/g 200

    Triglycerides 100 ®
     
  5. vkiernan

    vkiernan Silver

    Thanks Jansz. But what does it really tell me? Do I have to worry about Celiacs and what about the fat malabsorption???? I have to have a consult with Jack but until I do, I really don't know anything about this stuff. Looks like I don't have Candida though so thats positive.
     
  6. JanSz

    JanSz Gold

    I am not a doctor.

    Until you see dr Kruse, you may want to try:



    Eliminate all grains from your diet.

    -----------

    3 tablets 4x/day Mucos Wobenzym'N

    Garden of Life

    Wobenzym N (800 Tablets )



    -----------

    3 capsules per meal

    Bile_Acid_Factors

    http://www.jarrow.com/product/284/Bile_Acid_Factors



    ------------





    ..
     
  7. I have the same fat malabsorption issue and my doc recommended I take ox bile. 500mg is too much so I dropped to 250mg and that with a regular enzyme seems to be just right! This is the one I use:http://www.amazon.com/Ox-Bile-125-180-Capsules/dp/B003GHA5GS I found this to be a good one to play with small amounts and raise up if you need to.
     
  8. tweety

    tweety New Member

    Not to hijack, but Patty Cakes could you tell me the benefits you are experiencing from the ox bile? Do you also take HCL?



    I also have fat malabsorption but I do take HCL. I wondered recently about ox bile but hesitate to add anything else.
     
  9. Yes, Tweety I take both HCL and ox bile. I can't tell anything from just straight HCL. The HCL is for acid in the stomach to help digest protein. Then I added the ox bile at the high dose I got diarrhea so I knew something was working. But maybe a little too much! SO I went down to the 125mg size and started with one, then two and three (375mg). Sometimes when I eat a lot of fat I go more and sometimes I go less. I use my intuition on how much. So you may want to add just a small amount like the 125mg and see if you notice anything.
     
  10. tweety

    tweety New Member

    Ok, I will consider this in a couple of weeks. I have to go slower introducing more stuff bcs I tend to make a bunch of changes all at once, then never know why I am getting a particular reaction.



    VKiernan- I know you have not been able to use HCL lately- whatever happened with your acid stomach symptoms? Have you used ox bile as well?
     
  11. vkiernan

    vkiernan Silver

    No ox bile as my gallbladder is working fine according to all the tests I had done last fall. I was under the impression that ox bile is for those with no gallbladder. Could totally be wrong on that. Sounds like I need to look up ox bile and get better informed.



    As for the stomach acid stuff, had it thru the next day and then my stomach bloat went down and very little discomfort after that. Really have no idea why it came and why it left; just grateful it did. I'm guessing things are still changing from the seafood and from CT'ing. I have a messed up gut so I can only assume it will take some time.



    As for HCL, I cannot take that stuff. Gives me acid reflux or at least a week or so it did. Too afraid to try it again. I really took a lot before and now I can't take any. Too me that is a good sign. I was eating those bad boys like candy before up to about 10 to 15 a day.
     
  12. vkiernan

    vkiernan Silver

    How much HCL are you taking and when?
     
  13. vkiernan

    vkiernan Silver

    WHY do we have this problem? Any idea? Is it due to not the right gut flora? Can we fix it?
     

  14. Gut flora, diet, and of course the infamous, hormone imbalance. But I'm sure there is more..... I think we can fix it with "constant vigilance".
     
  15. vkiernan

    vkiernan Silver

    What was your number for the gluten issue? Do you have celiacs? I rarely eat any grains and don't know why my number is what it is. Maybe even rarely is too much. I might eat dairy a little in butter if we go out to eat but seldom grains unless I am getting it in something I am unaware of.
     
  16. vkiernan

    vkiernan Silver

    Jansz, thank you very much for responding. You have had succes then with what you recommended? I looked up the enzymes and holy crap are they expensive. But, if they work I am willing. No sense throwing more money down the toilet on stuff that doesn't work.;) I take quite a few NOW enzymes currently.
     

  17. I have never been diagnosed as celiacs and only stopped gluten because of the Hashis. I have not had gluten knowingly for about 18 months and zero grains for 8 months. I'm extremely strict about the grain issue. I also don't eat dairy unless I get it eating out. We rarely eat out except for sushi and most of it is raw and no butter there. My doc thought I need to check my skin care products and shampoo for wheat or wheat derivatives for exposure. I don't know where else I could be getting it unless some crumbs are getting in my food from my step-sons bread!!



    My number shocked me too! My Anti-gliadin was 7.4 marked as High. But I also showed Candida and h.pylori so my gut appears to have more issues that need to be resolved than yours.
     
  18. Destiny

    Destiny New Member


    Patty, you need to eradicate that h. pylori. According to dr. K, it has to go even if you have to use antibiotics. I had two triple therapies (mixture of antibiotics and prilosec) and one quadruple one. The last one got rid of it.
     
  19. vkiernan

    vkiernan Silver


    Damn that you have hashis. As far as I know, I don't. I have been tested twice. Not sure thats enough tests or not. So your number for the Agi was the same as mine too. That is strange for sure. I did have Candida according to blood tests twice but have done a lot of things to try to rid myself of it. It said none so I am assuming but can't verify until I talk with Jack. Perhaps that is why my FT3 is rising??? I have also read though when the FT3 is over the range and your TSH is low that is due to the adrenals not working well. I know I need to do an ASI but that is supposed to give you results over a 6 month time frame I believe so that would not necessarily tell me if I have made progress in the short term would it? I am more confused all the time.



    Now that I know you and I are both really in the same boat, I would like to compare more notes and what each of us is doing to help ourselves and the progress we are making. Might help us get there quicker. We are even the size basically. If you are game, pm me.
     
  20. vkiernan

    vkiernan Silver

     

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