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2015 Heteroplasmy bio hack results: nicotine and MB

Discussion in 'Redox Rx' started by Jack Kruse, Jan 27, 2016.

  1. Jack Kruse

    Jack Kruse Administrator

    What have I learned so far? Why are summer time high fat diets not a great thing to do chronically across all seasons? High fat diets force the use of mitochondria because they are oxidative powerhouses for fat. But what if you are designed to be loosely coupled by your mtDNA haplotype. If you have a Northern Europe haplotype you are loosely coupled. People who are loosely coupled are set up to have higher levels of mitochondrial heteroplasmy. As heteroplasmy increase disease phenotypes morph dramatically. But what if your mitochondria functionally suck = %heteroplasmy? If that is the case you must first optimize the things a mitochondria uses to recycle via mitophagy. Heteroplasmy implies altered O2 levels called pseudohypoxia and UV light assimilation via our surfaces to maintain NAD+ levels at cytochrome 1 NADH couple. What does that mean if you have a high percentage of heteroplasmy in your mitochondria? = real bad mito-nuclear coaptation = poor redox. What does it mean for good coaptation? It means you burn fat well and you need very little food to sustain yourself because you assimilate photons and electrons from the sun and magnetic field by grounding so well. High carb foods only grow in high UV environments so electrons from carbohydrates should carry higher photonic power. So how does this translate to biochemistry? High carbohydrate diets provide more energy by fermentation because of these highly powered electrons, without causing humans actually needing to use their badly functioning mitochondria. Bacteria tend to like this situation. Where are bacteria in your body? Every surface where you assimilate light. How to do bacteria and your mitonuclear coapatation work together? Here is clue: those with mitochondrial diseases who have "adopted, used, and advocated" long term ketogenic diets have been known to collapse into coma because their damaged mitochondria can't provide enough energy needed for a conscious state. When you have a high percentage of heteroplasmy ketosis can actually hurt you. This is not well appreciated by the LCHF food guru's. Mitochondria actually cannot function physiologically to generate energy for us neurologically without help from fermentation when heteroplasmy is elevated. Environmentally broken paleo advocates use and advocate carbs for that reason. Environmentally broken LCHF folks just get sicker as time evolves because they cannot replace mitochondria and/or DHA fast enough to offset their environment toxicity and they allow their mitochondria to face. Could we hack heteroplasmy? Yep. Some might begin to advocate temporary nicotine use when they are completely stalled and spinning their wheels. Why might nicotine work in this hack? What other cofactors would make nicotine work even better? Methylene blue could be a huge boost. Methylene blue is a MAO inhibitor and you learned about them in Time 6 and 7. MG can affect all the levels of endogenous biogenic amines. So if you are taking drugs that interact with this axis you need to be careful. MB can therefore can interact with selective serotonin reuptake inhibitor (SSRI) and MAO inhibitors to cause serious serotonin toxicity or serotonin syndrome. What about nicotine? Nicotine works best with good O2 consumption by mitochondria and strong natural full spectrum sunlight. Most nicotine users smoke so they never see the optimized effect of nicotine because of co-morbid lung disease from smoking. We can use a nicotrol inhaler to offset that risk. Most people who are sick rarely go out in the sun because their doctors or their beliefs keep them ignorant of basic physics. Going out in the sun makes us feel well because it causes release of beta endorphin from our skin. Runners get a high from this but the high is always greater in natural light. So how might nicotine work when O2 and UV light are optimized in your bio-hack? Nicotine slightly increases glucose levels to give a small superoxide pulse. This pulse make the perfect amount of singlet state ROS/superoxide to be the stimulus to lower the % of heteroplasmy in our cells. This small stimulus does two key things: 1. the small stimulus stimulates regeneration programs in us. 2. It stimulates mitophagy to recycle heteroplasmic poor functioning mitochondria to make new mitochondria and dig yourself out of the hole called mitochondria senescence. You cannot get the superoxide pulse without the O2 or the UV exposure. Did you know that DHA in your RBC's in skin arterioles 7-10 AM allows you to absorb even more UV light? This is possible because UVA light stimulates nitric oxide that vasodilators the skin arterioles to come closer to the surface so that UV light can be absorbed by porphyrins and hemoglobin in RBC's. UVB light penetrates only 0.1 mm. UVA penetrates 1-3 mm so to get UV light RBC's have to move toward the surface and they do because of the light nitric oxide couple. This is also why light makes plants move toward light too. If you're anemic, hypoxic, or diabetic, your fighting an uphill battle because your % of heteroplasmy is getting larger than it was when you were younger. Diseases can manifest just from the slow increase of heteroplasmy in mitochondria. But we can reverse the process if we get the natural details correct of how light works with chemicals in our body. Few people have that discipline in their bio-hacks. Some do. That is why we share what we have learned. All people as they age, they acquire this same kind of mito-nuclear coaptation dysfunction because as redox lowers, mitochondria uncouple and get more leaky and heteroplasmy increases. The major difference in aging compared to disease generation is that an aging person has a slower timescale of the development of heteroplasmy compared to those who develop illnesses at a younger age. The % of heteroplasmy is slower in natural aging, but faster in neolithic diseases in today's modern humans 20-40 years old. Their environment is driving the increased heteroplasmy. MB and nicotine can be used to combat this process.
     
  2. lilreddgirl

    lilreddgirl New Member

    What's a beginner's way to start using MB and nicotine to combat the process?
     
    Cpt.Tired likes this.
  3. Jack Kruse

    Jack Kruse Administrator

    there is none........emperic use and in most places you will need MD's help.

     
    Mystic Rose60 likes this.
  4. Josh Rosenthal

    Josh Rosenthal Charter member of Purple Angels Club

    Is there a clinical marker to determine the heteroplasmy?
     
    TerrierMom likes this.
  5. Lahelada

    Lahelada New Member

    Are you in a position to elucidate why you would want a physician in the loop or is the reason simply to establish the clinical markers of heteroplasmy? Or in other words are there downsides to MB you can elucidate on?
     
  6. Jack Kruse

    Jack Kruse Administrator

    not yet.......but this is why you need to watch Wallace video

     
  7. Lahelada

    Lahelada New Member

    Lol,and just today I was extolling the virtue of passing via B when trying to get to C...
    Will watch the video now.
    For info pharmaceutical MB is easy to find here.
     
  8. Josh Rosenthal

    Josh Rosenthal Charter member of Purple Angels Club

    (other than redox)
     
  9. Lahelada

    Lahelada New Member

    Thanks !
     
  10. Mystic Rose60

    Mystic Rose60 Let the sun shine on you :))

    I've listened to this video twice now and did more research on the use of MB in cancer, aging and dementia and based on my context/n=1 have made the decision to use it on a empiric basis now. I found a quality pharmaceutical source and it should be here tomorrow.
     
    SunnyDay, Martin and lilreddgirl like this.
  11. lilreddgirl

    lilreddgirl New Member

    @Mystic Rose60 what pharmaceutical source are you using and what dosage do you plan to test? :) researching more now myself...
     
  12. In the last century, new information about the effects of methylene blue has accumulated, making it possible to reinterpret former work on the subject, and the research directly guided by Ehrlich's ideas, including the discovery of the sulfa drugs, anti-inflammatory and antipsychotic drugs.

    Ideas of cellular coherence, polariz-ability of molecules and cells, liquid crystalline phase changes, and other non-local properties of molecular systems have established a basis for a complete revision of the "cell model" that dominated the 20th century.

    The lock-and-key idea, as applied to "receptors," enzymes, and antibodies, implies that a closely complementary spatial arrangement of specific atoms determines the biological effect. Several types of evidence show that there is more to cellular activation than a good spatial and chemical fit between effector and receptor. For example, Luca Turin (e.g., Franco, et al., 2011) has shown (in people and animals) that the way molecules vibrate governs the way they smell. Molecules with different shapes, but similar vibrations, smell the same, molecules with similar shapes, but different vibrations, don't smell the same. When deuterium (hydrogen that contains a neutron as well as a proton in its nucleus) is substituted in a molecule for hydrogen, the molecule has the same shape, but vibrates differently, and fruit flies can tell the difference by smell. They will reject a favored food when its molecules contain the heavy form of hydrogen. In Turin's view, such effects are transmitteed through proteins by alterations in the state of their electrons.

    Methylene blue is a potent cationic dye with maximum absorption of light around 670 nm. The specifics of absorption depend on a number of factors, including protonation, adsorption to other materials, and metachromasy - the formation of dimers and higher-order aggregates depending on concentration and other interactions:

    Species / Absorption peak / Extinction coefficient (dm3/mole·cm)
    MB+ (soln) 664 95000
    MBH2+ (soln) 741 76000
    (MB+)2 (soln) 605 132000
    (MB+)3 (soln) 580 110000

    Solutions of this substance are blue when in an oxidizing environment, but will turn colorless if exposed to a reducing agent. The redox properties can be seen in a classical demonstration of chemical kinetics in general chemistry, the "blue bottle" experiment. Typically, a solution is made of glucose (dextrose), methylene blue, and sodium hydroxide. Upon shaking the bottle, oxygen oxidizes methylene blue, and the solution turns blue. The dextrose will gradually reduce the methylene blue to its colorless, reduced form. Hence, when the dissolved dextrose is entirely consumed, the solution will turn blue again.
    In this sense, the home hack can be as simple as applying methylene blue solution (say 400mcg dissolved in a 20%ethanol solution, perhaps with *benzoic acid - a 1% solution) to the skin to test local tissue hypoxia. if the stain of the MB disappears within only a number of hours (say 4-8) you could say that the local tissue is hypoxic) This points to the property that MB can act as a substitute terminal electron acceptor in place of O2. This does not require the help of a doctor, as a lifetime supply of MB to be used topically can be purcahsed for about $8-21 at an aquarium store. Those withing to take it orally may want to cource pharmaceutical grade (i do not know where the differences lie, likely in purity)
    *benzoic acid had a number of cool properties. First, it uncouples mitochondria in extremely low concentrations (less than 1picoM) and that uncoupling capacity does NOT increase much with dosage. So, it has a very wide range of effectiveness and safety unlike drugs such as DNP which can kill you if you miscalculate the dosage just a bit. Benzoc acid also reduces ammonia in the body and stimulates the urea cycle. This property is helpful since many people suffer from ammonia overload cause by slow metabolism and stress. Finally, benzoic acid is very well absorbed topically and orally and is not known to affect negatively organs like the liver or the digestive system.

    topical use of MB: http://www.ncbi.nlm.nih.gov/pubmed/7708136

    So, Methylene blue can form a link between a cell's reductants, such as cysteine and glutathione, and its oxidants, including oxygen and hydrogen peroxide. By oxidizing cysteines (Crowe, et al., 2013), it can prevent the polymerization of microtubules.
    Microtubules, which make up the mitotic apparatus that separates the chromosomes and divides the cytoplasm when cells are proliferating, activate the enzyme that synthesizes nitric oxide. Nitric oxide blocks oxidative energy production, and activates the characteristic cancer metabolism, aerobic glycolysis (Brix, et al., 2012), creating the state of "pseudohypoxia."

    When an inflammation was created experimentally to test the ability of blood cells to transmigrate through endothelial cells, treatment with methylene blue increased the transendothelial movement of granulocytes, T- and B-lymphocytes, and monocytes, but it didn't have that effect on a T-cell line derived from a person with leukemia (Werner, et al., 2013), showing that it was a selective process, rather than just an increased permeability. An excess of nitric oxide increases general permeability of blood vessels.

    Methylene blue, by its ability to be reduced and oxidized, can restore respiration to cells whose mitochondria have been damaged in various ways, even by cyanide (Barron, 1930). That's involved in its recognized use to treat shock, correcting the inability of mitochondria to use oxygen. Nitric oxide blocks the use of oxygen in shock, and methylene blue blocks the synthesis of nitric oxide, while at the same time it activates mitochondrial energy production. Besides the restoration of cellular respiration, methylene blue probably acts more directly on cellular structure and sensitivity, for example by oxidizing sulfhydryl groups that regulate the "excitatory amino acid receptor," providing protection against excitotoxity, again functioning in direct opposition to nitric oxide.

    Methylene blue is being investigated as a treatment for the major brain degenerative diseases, psychoses, anxiety, depression, and cancer. In a small daily dose of 15 milligrams, in a well controlled study, it was effective against severe depression (Naylor, et al., 1987), and many animal studies show that it is effective against anxiety. There are probably many things involved in these effects on the nervous system--the anti-excitatory action, a variety of anti-estrogen effects, and increase of thyroid hormone while decreasing pituitary hormones--but they all seem to involve protection against nitric oxide.

    Over-production of nitric oxide is involved in many of the symptoms of viral infections, including herpes, and the therapeutic effects of methylene blue suggests that natural resistance to viral infections might depend on maintaining a safely oxidizing redox balance.

    Reversal of cancer metabolism by methylene blue (Poteet, et al., 2013) is another of its effects that probably involves reversal of the effects of nitric oxide, since nitric oxide promotes aerobic glycolysis and tumor growth (Caneba, et al., 2014).
    SUMMARY:

    Methylene blue has the properties of DISASSOCIATING NO from Cytochrome C Oxidase in a similar way to red light (no surprise given its absorption spectrum) as well as inhibiting the synthesis of new NO molecules, AND taking the place of oxygen as a terminal electron acceptor, essentially transporting electrons directly from complex 1 to CCO and in the process creating no ROS. this is essentially clean efficient electron transfer with no ill effects at correct dosage.

    Again something like 400-800mcg in solution as mentioned above taken orally should show no negative effects, but topical absorption (whilst leaving a stain) has been shown to have excellent properties.

    If people are worried about serotonin syndrome taking MB with caffiene should mitigate any effects due to its dopaminergic properties. or you can take microdose LSD if you are so inclined :alien:

    Other:
    http://selfhacked.com/2013/08/25/methylene-blue-the-cheapest-cognitive-enhancer/
     
    Last edited: Jan 28, 2016
    rlee314, Paul T., Pebbles and 12 others like this.
  13. lilreddgirl

    lilreddgirl New Member

    http://nutritionreview.org/2015/12/...-aging-treats-progeria-symptoms-in-new-study/

    Methylene Blue Reverses Human Cell Aging, Treats Progeria Symptoms in New Study

    by NUTRITONREVIEW.ORG on DECEMBER 15, 2015 · 0 COMMENTS

    in AGING, NEWS

    [​IMG]University of Maryland (UMD) researchers report that methylene blue (MB), a common chemical with a 120-year-long history of diverse applications, can treat progeria, a rare genetic disease that imitates the body’s aging process at an augmented rate. Lab test found that small doses of methylene blue can almost fully repair progeria-damaged cells.

    The study also revealed that methylene blue can treat age-related damage in healthy cells, suggesting that methylene blue can also treat normal aging symptoms.

    In recent years there has been a surge of interest in MB as an antimalarial agent and as a potential treatment of neurodegenerative disorders such as Alzheimer’s disease (AD), possibly through its inhibition of the aggregation of tau protein.

    Progeria symptoms manifest within a person’s first year of life. This rare genetic disorder causes the skin to thin and wrinkle as well as joints and bones to become fragile. Other symptoms include organ failure and body hair loss. Majority of progeria patients do not reach adulthood and can barely survive their teenage years.

    [​IMG]“It seems that methylene blue rescues every affected structure within the cell. When we looked at the treated cells, it was hard to tell that they were progeria cells at all. It’s like magic,” said senior study author Kan Cao, a molecular genetics and cell biology associate professor at UMD.

    The rare genetic disease is caused by a single defect in a gene that produces a type of protein called lamin A. Normal, healthy cells cut a small piece in every lamin A produced. This minor tweak enables the lamin A to work effectively.

    Progeria-defected cells sidesteps the minor tweak. The lamin A becomes defective and blocks the nuclear membrane. This results in cluster formations and distortions that interfere with normal body functions.

    Cells inflicted with progeria contain defective and distorted mitochondria that produce a cell’s energy. The researchers found that most mitochondria in progeria-inflicted cells are jagged and inflated. This disrupts the mitochondria’s main function.

    In the lab tests, methylene blue reverses the damages progeria had inflicted to both mitochondria and nucleus. The resulting progeria cells became almost identical to healthy cells.

    When methylene blue was tested in healthy cells that were allowed to progress at the normal rate, the chemical also repaired the age-related damages. The findings were published in the Aging Cell journal on Dec. 10.

    “This is such an exciting result with so much potential, both for progeria and normal aging,” added lead author Zheng-Mei Xiong, a postdoctoral associate at UMD.

    Methylene blue’s ability to repair cell defects can pave the way for full-body treatment in progeria patients. Findings provide a basis of methylene blue’s wide-spread use in the cosmetic and nutritional supplement industries as an anti-aging additive.

    Source: http://onlinelibrary.wiley.com/doi/10.1111/acel.12434/abstract
     
    Cpt.Tired, lohd2015, seanb4 and 2 others like this.
  14. im gonna go drink my daily dose right now :whistle:
     
  15. Mystic Rose60

    Mystic Rose60 Let the sun shine on you :))


    Hi lilredgirl, I ordered from Bluebrainboost and it actually arrived today. As far as the dose, I'm no sure yet. I'm still doing my research .
    https://www.bluebrainboost.com/p/methylene-blue-nootropic/

    Alternative mitochondrial electron transfer as a novel strategy for neuroprotection.
    http://www.ncbi.nlm.nih.gov/pubmed/21454572

    Methylene blue facilitates the extinction of fear in an animal model of susceptibility to learned helplessness
    http://www.sciencedirect.com/science/article/pii/S1074742706001079

    Methylene blue restores spatial memory retention impaired by an inhibitor of cytochrome oxidase in rats.
    http://www.ncbi.nlm.nih.gov/pubmed/12384216

    Methylene blue improves brain oxidative metabolism and memory retention in rats.
    http://www.ncbi.nlm.nih.gov/pubmed/14724055

    STRIATAL NEUROPROTECTION WITH METHYLENE BLUE
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2765788/?tool=pmcentrez

    One person shared their personal experience with MB
    My Experience with Methylene Blue

    "My first day: I bought Methylene blue from the aquarium last night. Prepared it, a 60 microgram solution, its very easy thing to do , you just need a eye dropper, a few cups and a measuring glass.

    When I took it I felt great energy. My thoughts were easily facilitated, it was as if I got the stereotypical "this is how I am usually supposed to feel" feeling. Which this could actually be the case because its increasing metabolism in the places of your brain that need it.

    This nootropic has a different feel to it. I can say it definitely takes away social anxiety no problem, because it feels like your young again, where your whole world is focused on what's in front of you. That was the biggest effect I noticed. The second biggest thing I noticed, is the learning after the fact process. I could say that MB does help with thinking while doing a task due to the relaxed nature of how you feel which I just described as reduced social anxiety, but I would say that the biggest nootropic effect is after the fact learning. I felt when I would read or do something, after I am done, I feel like I fully understand the subject thoroughly and can implement it from there on out, whether it is a life lesson or an academic lesson or even a logical lesson. I would say that methylene blue facilitates the idea "fool me once shame on you, fool me twice shame on me" but without the fool me twice, because the MB facilitated the full understanding of the subject after the first try. Sort of..kind of..like selectively putting what is in your long term memory. Ask me more questions, if you ask me a question Ill be able to answer it instead of me just rambling. MB also definetly increases the energy department, I have in the past always felt tired when I knew I shouldn't or I would get tired much faster than other people, and I feel like MB kicks that completely out the door. Not only do you get the energy from optimal mitochondrial processing, but I feel like most people get tired quickly due to stress, and it seems as if it eliminates stress (or makes stress not seem as monumental as people can make it) thus facilitating more energy with a relaxed state of mind.

    There are more effects I will continue to right about when I come across them again. Im taking 60 micrograms right now each dosing, and I dose periodically, mostly 4 hours apart, we'll see what happens. "

    I'll share more when I come back. Enjoy!:)
     
  16. Dia

    Dia Gold

    I've actually been biohacking MB after reading Time 6, since last week. Great stuff! Just an overall feeling of well being, clarity, cognitive improvement ect. My body actually feels like I just had a great massage or when I have a good epsom salt bath, for hours after use. I found I get better results with it on the days I do the D lamp though.
     
  17. this is what correct speedy metabolism provides folks.
     
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  18. Dia

    Dia Gold

    I did also found that I felt better when I had a coffee with it. I like it without the coffee, but with coffee,I was really mellow.
     
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  19. Dia

    Dia Gold

  20. Dia

    Dia Gold

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